Harlequin frog is a non-scientific name for any of several varieties of frog or toad, including the following:
- Atelopus Atelopus is a large genus of true toads natively distributed in Costa Rica to Bolivia and French Guiana. Atelopus are generally brightly colored and diurnal. Most species are associated with mid- to high-elevation streams. This genus has been greatly affected by amphibian declines, and many species are now considered endangered, while others, a genus of South American toads
- Harlequin Poison Frog (Dendrobates histrionicus), a species of South American poison dart frog
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